pbmn mcherry parkin (Addgene inc)
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pbmn mcherry parkin
Pbmn Mcherry Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbmn+mcherry+parkin/pBMN-mCherry-Parkin+(Plasmid+%2359419)/pmc12759083-20-0-2
Average 93 stars, based on 13 article reviews
Pbmn Mcherry Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbmn+mcherry+parkin/pBMN-mCherry-Parkin+(Plasmid+%2359419)/pmc12759083-20-0-2
Average 93 stars, based on 13 article reviews
pbmn mcherry parkin - by Bioz Stars,
2026-09
93/100 stars
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other:Article Title: A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery Article Snippet: pLV-mitoGFP was a kind gift from Polymerase Chain Reaction:Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: To generate mCherry-Parkin and BFP-mito lentiviruses for cell line generation 4E6 HEK293T cells were seeded into 10 cm plates and transfected the next day with 45 μL Mirus LT1 transfection reagent (MIR2300) added to a mixture of 5 μg each (15 μg total) of plasmids VSV-G (addgene: 8454), R8.74 (addgene: 22036) or CMV-Gagpol (addgene: 35614), and one of the following: pLV-mCherry-Parkin (this study), pLV-BFP-mito (this study), and Su9-GFP-HALO (addgene: 184905) in 1.5 mL Optimem (ThermoFischer catalog: 31985062) according to manufacturer recommendations. .. Both lentivirus plasmids were restriction subcloned using NheI and BsrGI into pMK1253 (addgene: 133058) from Amplification:Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment Article Snippet: To generate mCherry-Parkin and BFP-mito lentiviruses for cell line generation 4E6 HEK293T cells were seeded into 10 cm plates and transfected the next day with 45 μL Mirus LT1 transfection reagent (MIR2300) added to a mixture of 5 μg each (15 μg total) of plasmids VSV-G (addgene: 8454), R8.74 (addgene: 22036) or CMV-Gagpol (addgene: 35614), and one of the following: pLV-mCherry-Parkin (this study), pLV-BFP-mito (this study), and Su9-GFP-HALO (addgene: 184905) in 1.5 mL Optimem (ThermoFischer catalog: 31985062) according to manufacturer recommendations. .. Both lentivirus plasmids were restriction subcloned using NheI and BsrGI into pMK1253 (addgene: 133058) from Infection:Article Title: Phase II trial of cytarabine and mitoxantrone with devimistat in acute myeloid leukemia Article Snippet: Antibodies against TOM20 (Cell Signaling, #42406; 1:1000), VDAC (Abcam, ab14734; 1:1000), and β-actin (Abcam, ab8227; 1:2000) were used. .. MFL2 cells were infected with Plasmid Preparation:Article Title: Phase II trial of cytarabine and mitoxantrone with devimistat in acute myeloid leukemia Article Snippet: Antibodies against TOM20 (Cell Signaling, #42406; 1:1000), VDAC (Abcam, ab14734; 1:1000), and β-actin (Abcam, ab8227; 1:2000) were used. .. MFL2 cells were infected with Article Title: Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy. Article Snippet: .. pRK5-Myc-Parkin (Myc-Parkin) plasmid was a gift from Ted Dawson (Addgene plasmid # 17612); pM2.G and psPAX2 vectors were a kind gift from Didier Trono (Addgene plasmid #12259 and #12260); pET28a/6His-GST-3C-Parkin (GST-Parkin) was a gift from David Komander (Addgene plasmid #110758); pCMV6/Myc-Mul1 (Myc-Mul1) was purchased from Origene. pEGFP-N2/MARCH5 (GFPMARCH5) plasmid was a kind gift of Eric Schirmer (Addgene plasmid #62039). pEGFP-parkin WT (EGFP-Parkin) plasmid was a gift from Edward Fon (Addgene plasmid # 45875); pcDNA3.1/Myc-His-Mfn2 (Myc-Mfn2) plasmid was a gift from David Chan (Addgene plasmid # 23213). Generated:Article Title: Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy. Article Snippet: .. pRK5-Myc-Parkin (Myc-Parkin) plasmid was a gift from Ted Dawson (Addgene plasmid # 17612); pM2.G and psPAX2 vectors were a kind gift from Didier Trono (Addgene plasmid #12259 and #12260); pET28a/6His-GST-3C-Parkin (GST-Parkin) was a gift from David Komander (Addgene plasmid #110758); pCMV6/Myc-Mul1 (Myc-Mul1) was purchased from Origene. pEGFP-N2/MARCH5 (GFPMARCH5) plasmid was a kind gift of Eric Schirmer (Addgene plasmid #62039). pEGFP-parkin WT (EGFP-Parkin) plasmid was a gift from Edward Fon (Addgene plasmid # 45875); pcDNA3.1/Myc-His-Mfn2 (Myc-Mfn2) plasmid was a gift from David Chan (Addgene plasmid # 23213). Mutagenesis:Article Title: Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy. Article Snippet: .. pRK5-Myc-Parkin (Myc-Parkin) plasmid was a gift from Ted Dawson (Addgene plasmid # 17612); pM2.G and psPAX2 vectors were a kind gift from Didier Trono (Addgene plasmid #12259 and #12260); pET28a/6His-GST-3C-Parkin (GST-Parkin) was a gift from David Komander (Addgene plasmid #110758); pCMV6/Myc-Mul1 (Myc-Mul1) was purchased from Origene. pEGFP-N2/MARCH5 (GFPMARCH5) plasmid was a kind gift of Eric Schirmer (Addgene plasmid #62039). pEGFP-parkin WT (EGFP-Parkin) plasmid was a gift from Edward Fon (Addgene plasmid # 45875); pcDNA3.1/Myc-His-Mfn2 (Myc-Mfn2) plasmid was a gift from David Chan (Addgene plasmid # 23213). Clone Assay:Article Title: Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy. Article Snippet: .. pRK5-Myc-Parkin (Myc-Parkin) plasmid was a gift from Ted Dawson (Addgene plasmid # 17612); pM2.G and psPAX2 vectors were a kind gift from Didier Trono (Addgene plasmid #12259 and #12260); pET28a/6His-GST-3C-Parkin (GST-Parkin) was a gift from David Komander (Addgene plasmid #110758); pCMV6/Myc-Mul1 (Myc-Mul1) was purchased from Origene. pEGFP-N2/MARCH5 (GFPMARCH5) plasmid was a kind gift of Eric Schirmer (Addgene plasmid #62039). pEGFP-parkin WT (EGFP-Parkin) plasmid was a gift from Edward Fon (Addgene plasmid # 45875); pcDNA3.1/Myc-His-Mfn2 (Myc-Mfn2) plasmid was a gift from David Chan (Addgene plasmid # 23213). |